International Journal of Nutrition and Food Sciences
Volume 4, Issue 5, September 2015, Pages: 530-534

Preservation of Sugarcane Juice Using Herbal Clarificant

Mst. Kohinoor Begum, Md. Shamsul Arefin, Md. Shariful Islam, Md. Jahirul Islam

Bangladesh Sugarcane Research Institute, Pabna, Bangladesh

Email address:

(Mst. K. Begum)

To cite this article:

Mst. Kohinoor Begum, Md. Shamsul Arefin, Md. Shariful Islam and Md. Jahirul Islam. Preservation of Sugarcane Juice Using Herbal Clarificant. International Journal of Nutrition and Food Sciences.Vol.4, No. 5, 2015, pp. 530-534. doi: 10.11648/j.ijnfs.20150405.13

Abstract: An experiment was carried out at Physiology and Sugar Chemistry Laboratory of Bangladesh Sugarcane Research Institute Ishurdi, Pabna, Bangladesh during 2013-2014 to determine the self life of sugarcane juice without adding any preservatives. Sugarcane varieties Isd 34, Isd 35, Isd 36, Isd 37 and Isd 38 were used for collection of juice and juice were collected in the month of September, October, November, December, January, February and March. Juice collected in the month of December and January showed the highest shelflife by using Mucilage substances of ladies finger (Hibiscus esculentus Linn) as a clarificant. Juice collected in the month of December, January showed highest self life due to presence of lower reducing sugar and highest percentage of sucrose.

Keywords: Sugarcane Juice, Preservation Technique, Clarificant, Self Life

1. Introduction

Sugarcane is an important industrial crop cultivated in tropical and subtropical regions of the world. Sugarcane has been used as a sweetener for millennia and today refined sugar is used in copious quantities to supplement the natural sugar (fructose) found in fruits and vegetables (Phanikumar, 2011). It possesses therapeutic value (Banerji et al., 1997).

Sugarcane juice is commonly used as a delicious drink in both urban and rural areas. Sugarcane juice of 100 ml provides 40 Kcal of energy, 10 mg of iron and 6 μg of carotene (Par­vathy, 1983). Sugarcane juice is rich in enzyme and has many medicinal properties. It contains water (75%-85%), reducing sugar (0.3-3.0%), non-reducing sugar (10-21%) (Swamina­than, 1995). Sugarcane juice is a great preventive and healing source for sore throat, cold and flu. It has a low glycemic index which keeps the body healthy. Even the diabetic can enjoy this one sweet drink without fear. It is because it has no simple sugars. It hydrates the body quickly when exposed to prolong heat and physical activity. It is excellent substitutes for aerated drinks and cola; it refreshes and energizes the body instantly as it is rich in carbohydrates. Sugarcane juice is also a popular drink in Bangladesh, but the cane juice is prepared in a unhygienic way. If the cane juice is collected, preserved in a hygienic way then they can be sold after short / long times preservation. On the other hand, it will meet up the nutritional problem of our large population.

In general sugarcane juice is spoiled quickly by the pres­ence of simple sugars. The sugarcane juice can be introduced as delicious beverages by preventing the spoilage of juice with appropriate method. Biodegradation is caused by microorgan­isms mainly Leuconostoc sp. (L. mesenteroides and L. dextra­nium) also takes place. Soon after the harvest of sugarcane; endogenous invertase enzyme is activated and acts as a cause of deterioration. These organisms convert sucrose into poly­saccharides, such as dextran. Besides, loss of sucrose, the pres­ence of dextran even in very small amount creates problem of filtration, clarification, crystallization and alters the shape of sugar crystals thereby affecting the quality of sugar (Krishna­kumar and Devadas, 2006).

Considering these facts, the present study has been planned to establish a protocol for preservation of sugarcane juice without adding any preservatives.

2. Materials and Methods

Many commercial juices are filtered to remove fiber. Common methods for preservation and processing of fruit juices include canning, pasteurization, freezing, evaporation, drying and addition of preservatives. A different method was followed for preservation of cane juice.

2.1. Collection of Sugarcane Juice

Six sugarcane varieties viz. Isd 34, Isd 36, Isd 37, Isd 38, Isd 39 and Isd 40 were used. 11 months aged canes were collected from the field. Then they were washed and collect the juice using a hand crusher.

Fig. 1. Ladies finger garden. Fig. 2. Mucilage substances collected from ladies finger.

Fig. 3. Prepared Mucilage substances collected from ladies finger. Fig. 4. Mixing the extract with boiling Juice.

Fig. 5. Preserved sugarcane juice in glass and plastic bottles after four months.

2.2. Preparation of Ladies Finger Extract and Mixing into Juice

First we collected the ladies finger plant. We cut the stem into pieces. Then we collect the outer layer of the stem and put into water for 30 minutes. Mucilage substances came out from stem and mixed with water. 250-300 gm extracts are used as clarificant for 180 liter sugarcane juice. Juices were then boiled at 1000C for 10 minutes. During boiling mucilage substances which were collected from ladies finger were mixed with juice. After mixing the extract all the dirty substances and fiber became clod together. Then we removed them completely. Thus we got a clear and transparent juice. Then the juices were bottled after cooling in germfree airtight bottles using laminar flow chamber and preserved for four months in room temperature (25 0 C).

2.3. Analysis the Juice Before and After Preservation

Juices were analysed before preservation and every 15 days after one bottle were analyzed for determination of qualitative characters like bix, pol, purity, reducing sugar. Microbial tests were also done after every 15 days.

2.4. Determination of Brix, Pol, Purity and Reducing Sugar

Soluble solids (0Brix) was determined by Brix hydrometer standardized at 20°C and Horne’s dry lead method was used for sucrose determination using an automatic polarimeter (Bellingham and Stanley ADP-220®). Juice purity was calculated as the ratio of the sucrose content and corrected Brix reading. Reducing sugars were determined by the method described in Queensland Laboratory Manual [Bureau Sugar Experiment Stations (BSES), 1970].

2.5. Statistical Analysis

The collected data for a character under certain treatment were calculated and statistically analyzed following Factorial Completely Randomized Design (CRD). The analysis of variance was performed and means were compared by Duncans New Multiple Range Test (DMRT) at 5% level of probability for interpretation of results.

3. Results and Discussion

3.1. Difference Between the Fresh Juice and the Juice After Boiling with Clarificant

Color of the fresh juice after collection were greenish but after boiling mixing with clarificant it became slightly reddish and brix percentage became increased 1-2%.

3.2. Qualitative Analysis Result Before and After Preservation

Before preservation Varieties have significant effects on different qualitative parameters of cane juice. Highest pol (14.05) and purity (88.28) percentage and lowest reducing sugar percentage (0.1833) were achieved in the variety Isd 38 followed by Isd 39 though it achieved highest brix percentage. The lowest brix (16.68), pol (11.56), purity percentage (84.98) and highest reducing sugar (0.5567) were observed by the variety Isd 34 (Table 1).

Table 1. Effects of varieties on different qualitative parameters of cane juice.

Name of varieties Brix Pol Purity R. S.
Isd 34 (V1) 16.68 e 11.564c 84.98b 0.5567 a
Isd 36 (V2) 18.78 d 12.588b 86.84 ab 0.4767 b
Isd 37 (V3) 19.73 c 13.487a 86.10 ab 0.4150 c
Isd38 (V4) 20.19 b 14.05 a 88.28 a 0.1833
Isd 39 (V5) 20.42 a 14.02 a 87.36 a 0.2683
Isd 40 (V6) 19.68 c 13.49 a 87.29 a 0.3800d
LSD (5%) 0.0640 0.7282 2.2615 0.0341

Table 2. Effects of months on different qualitative parameters of cane juice.

Name of months Brix Pol Purity R. S.
October (M1) 19.17 c 13.10 b 85.57 b 0.4450 b
November (M2) 20.08 b 13.85 a 88.02 a 0.1983 d
December (M3) 18.48 f 12.47 b 85.89 ab 0.6383 a
January (M4) 18.85 e 12.85 b 86.54 ab 0.2000 d
February (M5) 19.82 b 13.93 a 87.48 ab 0.3300 c
March (M6) 19.09 d 13.01 b 87.36 ab 0.4683 b
Lsd (5%) 0.0640 0.7282 2.2615 0.0341

Different months have significant effects on different qualitative parameters of cane juice. Highest pol (13.93) was achieved in the month February followed by November (13.85). Highest brix (20.08), purity percentage (88.02) and lowest reducing sugar (0.1983) were achieved in November (Table 2).

Interaction of variety with months have significant effects on different qualitative parameters of juice. Highest brix percentage was achieved by the variety Isd 40 in the month of February. In case of pol percentage highest level were achieved by the interaction of Isd 36 and Isd 39 with January, Isd 39, Isd 40 with the month of February and Isd 38 in the month of March. Highest purity percentage were observed by the varieties Isd 36 and Isd 40 in the month of October, Isd 34, Isd 36, Isd 39 and Isd 40 in the month of January Isd 38 in the month of February and March and Isd 39 in the March. Lowest reducing sugar was observed in Isd36 in the month of January and Isd 38 in the month of March.

Table 3. Interaction effects of Varieties x Months.

Varieties X Months Brix Pol Purity R. S.
V1M1 15.5 s 9.98 k 82.58 bcd 0.72 b
V2M1 19.4 j 13.42 a-h 88.71 a 0.35 fghd
V3M1 16.2 r 10.80 jk 84.94 abcd 0.66 bc
V4M1 15.2 t 9.58 k 80.26 d 0.38 efg
V5M1 16.9 q 11.14 i-k 84.50 abcd 0.58 c
V6M1 16.9 q 11.13 i-k 88.91 a 0.65 bc
V1M2 19.1 kl 13.11 a-h 86.34 ab 0.59 cd
V2M2 19.8 h 13.67 a-g 87.93 ab 0.30 gh
V3M2 17.5 p 11.80 h-j 85.89 abc 0.60 cd
V4M2 18.5 n 12.65 d-i 87.08 ab 0.30 gh
V5M2 19.5 ij 11.91 g-j 87.23 ab 0.44 e
V6M2 18.3 o 12.39 e-j 86.58 ab 0.63 c
V1M3 20.0 g 13.71 a-f 80.50 cd 0.54 d
V2M3 20.1 fg 13.66 a-g 87.36 ab 0.18 jk
V3M3 19.6 i 13.35 a-h 86.79 ab 0.54 d
V4M3 19.5 ij 13.31 a-h 86.97 ab 0.27 hi
V5M3 20.0 g 13.79 a-f 87.85 ab 0.43 ef
V6M3 19.2 k 13.10 a-h 87.15 ab 0.53 d
V1M4 20.2 f 14.02 a-f 88.52 a 0.33 gh
V2M4 21.0 bc 14.76 a 89.40 a 0.07 m
V3M4 20.1 fg 13.82 a-f 87.56 ab 0.27 hi
V4M4 19.0 lm 13.00 a-h 87.16 ab 0.08 lm
V5M4 20.9 c 14.60 a 88.87 a 0.15 jklm
V6M4 20.5 e 14.08 a-e 88.19 a 0.20 ij
V1M5 20.7 d 14.33 a-d 87.84 ab 0.15 jklm
V2M5 20.2 f 13.77 a-f 86.83 ab 0.15 jklm
V3M5 19.0 lm 12.76 b-i 85.53 abcd 0.87 a
V4M5 20.2 f 14.01 a-f 88.37 a 0.10 klm
V5M5 21.1 b 14.59 a 87.98 ab 0.18 jk
V6M5 21.3 a 14.65 a 87.61 ab 0.16 jkl
V1M6 19.5 ij 13.42 a-h 87.64 ab 0.34 gh
V2M6 20.0 g 13.80 a-f 87.90 ab 0.14 jklm
V3M6 18.5 n 12.29 f-j 84.65 abcd 0.89 a
V4M6 20.7 d 14.52 a 89.37 a 0.07 m
V5M6 20.5 e 14.22 a-d 88.47 a 0.20 ij
V6M6 18.9 m 12.69 c-i 85.69 abcd 0.64 bc
LSD (5%) 0.1567 1.7836  5.5395  0.0835

Lower level of pol percentage and higher level of reducing sugars (simple sugar) were observed in juice collected from the varieties Isd 34, Isd 37, Isd 38, Isd 39 and Isd 40 in the month of October and November. Higher pol% and lower reducing sugar was observed in Isd 36 from the month of October to March. Same trend was observed in varieties Isd 34 from the month of November to March. Preserved juice collected from variety Isd 36 in October to March and from varieties Isd 34, Isd 36 in November to March was lasted for four months. All the varieties showed higher pol percent and lower reducing sugar in the month of December to March. Preserved juice became spoiled within one week after preservation where comparatively lower pol percentage and higher reducing sugar were observed.

Results of qualitative parameter remain same after four months for juices which were preserved in the month from December to march. Preserved juices were also free from any microbial contamination.

High temperature, high humidity and rainfall are responsible for spoiling the cane juice. In Bangladesh temperature and humidity began to fall from October. In the month of December and January Lowest temperature and humidity was observed. If we preserved juice in December and January it will lasting for four months without deteriorating its quality because sucrose cannot convert into reducing sugar due to low temperature. Sucrose itself a good preservative and preservation capacity of sucrose is better than other monosaccharide like glucose and fructose which are known as reducing sugar. The reducing sugars in juice are an important indicator of cane deterioration (Uppal and Sharma, 1997; Magdum et al., 1987; Ahmad and Khan, 1988; Gaur and Desai, 1988). Bhatia et al., (2009) have reported a gradual increase in titrable acidity with parallel decline in pH of juice was also observed in all the genotype during storage (Bhatia et al., 2009). However, this effect was more pronounced during late crushing period i.e. at high temperature as reported by Mao et el., 2006. Uppal et al., (2006) have also observed decreasing trend of purity coefficient with increasing storage periods. Steel and Trost (2006) reported that the presence of bacteria reduced the sugar purity, which may be another reason for reduction of purity. The ambient temperature rises beyond 350C, and then a water soluble polymer of glucose (dextran) is synthesized from the stored sucrose. The dextran content in stale cane juice increased sharply with increase in dextransucrase activity. An enzyme extracellularly secreted by Leuconostoc bacteria present in rhizophere. The cut ends of the harvested cane facilitates invasion of microbes, particularly Leuconostoc bacteria inside cane, which converts stored sucrose into dextran through dextransucrase enzyme (Kin and Robyt, 1995). Luzio and Mayer (1983) reported that dextransucrase catalysed the hydrolysis of the substrate (sucrose) and that a glucosylated enzyme had three competing activities, hydrolysis, D- glucosyl transfer and polymerization. This enzyme secreted mostly by Leuconostoc bacteria, not only catalyses dextran synthesis from sucrose, but in the presence of other carbohydrates such as glucose, fructose also transfer glucose from the sucrose molecule to form oligosaccharides such as Leucrose and Palatinose (Robyt, 1995; Robyt and Eklund, 1982) and therefore, is a potential criteria for juice deterioration (Eggleston and Legendre, 2003). Through boiling the juice at 1000C for ten minutes, can reduced bacterial activities.

Considering the different factors juice collected from different varieties in the month of December and January showed higher lasting period using mucilage substance of ladies finger as clarificant.


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